解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Several purified species of human leukocyte interferon, including recombinant interferons, inhibit the multiplication of tobacco mosaic virus (TMV) in tobacco protoplasts derived from various cultivars. Viral RNA accumulation was determined by dot-blot hybridization to specific cDNA probes, and virus antigen was deter...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90456-8
更新日期:1985-01-15 00:00:00
abstract::Published ultraviolet (uv) inactivation data and in vitro transcription studies have suggested that vesicular stomatitis virus (VSV) leader RNA was solely responsible for the inhibition of host cell RNA synthesis by this virus. Since no protein product is encoded in leader RNA, this conclusion implied that no protein ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90448-9
更新日期:1985-01-15 00:00:00
abstract::Pea enation mosaic virus (PEMV) RNA was isolated from virions and then translated in rabbit reticulocyte lysates and in wheat germ extracts. RNA 1 (apparent molecular weight, Mr 1.77 x 106) was shown to code for two major translation products, vp2 (Mr 88,000) and vp4 (Mr 36,000) plus a high-molecular-weight minor prod...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90369-6
更新日期:1984-12-01 00:00:00
abstract::Eight different human rotavirus strains were adapted to replicate in cell culture. In vitro, rotavirus genetic reassortment has been performed between two virus strains, one with a long pattern of segmented genomic RNAs and the other with a short pattern in the electrophoretic mobility of genomic RNAs. This genetic re...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90381-7
更新日期:1984-12-01 00:00:00
abstract::Antigenic variation among influenza B viruses is different from that of influenza A in several ways. Antigenic shift has not been observed, distinct antigenic variants of influenza B cocirculate, and antigenically similar viruses have been isolated many years apart. To study the mechanism of antigenic drift in influen...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90384-2
更新日期:1984-12-01 00:00:00
abstract::Particles purified from the liver of hepatitis B virus-infected patients were previously shown by us to incorporate 32P-deoxynucleotides into viral DNA and DNA-RNA hybrid molecules when incubated in a DNA polymerase reaction mixture. In this investigation, similar particles from duck and ground squirrel livers infecte...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90330-1
更新日期:1984-11-01 00:00:00
abstract::From two human brain tumors SV40 genomes were isolated by recombinant DNA techniques. The SV40 genome cloned from a human meningioma DNA was shown to be indistinguishable from wild-type SV40. In contrast, the SV40 genome cloned from a human astrocytoma proved to be a nonviable deletion mutant with a truncated early re...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90357-x
更新日期:1984-10-30 00:00:00
abstract::A full-length human endogenous provirus termed ERV3 was isolated from a human fetal recombinant DNA library by low stringency hybridization with two probes: baboon endogenous virus LTR; and a pol-env subclone from the endogenous chimpanzee provirus, CH2. DNA sequencing within the clone and comparisons with other retro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90347-7
更新日期:1984-10-30 00:00:00
abstract::Monoclonal antibodies were used to compare the antigenicities of the haemagglutinins of two receptor binding mutants of X-31 (H3N2) influenza virus. The mutants which differed from each other in recognizing sialic acid in either alpha 2-6 linkage or alpha 2-3 linkage to galactose also differed exclusively at residue 2...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90158-2
更新日期:1984-10-15 00:00:00
abstract::The genes of the RF strain of bovine rotavirus have been cloned into pBR 322 following the synthesis and hybridization of cDNA transcribed from both strands of in vitro polyadenylated genomic RNA. Cloned rotavirus DNAs were assigned to most of the 11 genomic RNA segments by Northern blot hybridization. The complete se...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90159-4
更新日期:1984-10-15 00:00:00
abstract::It has previously been shown that a far-upstream region of the herpes simplex virus immediate early (IE) gene 3 increases the expression of a linked thymidine kinase (TK) gene and also contains sequences which respond to stimulation of transcription by a virion component. The effects of altering the orientation and di...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90238-1
更新日期:1984-09-01 00:00:00
abstract::Two temperature-sensitive mutants of herpes simplex virus type 1 in complementation group 1-1 were analyzed to determine if the major DNA-binding protein they produced was thermolabile. Cells infected with these mutants were analyzed for deoxyribonucleoprotein complexes containing the DNA-binding protein. These comple...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90220-4
更新日期:1984-09-01 00:00:00
abstract::A new mutation designated sur was isolated as a suppressor of a mutation in the uvsX gene of T4 phage. Unlike the other suppressors of mutations in genes involved in DNA repair and recombination, sur has a wide range, suppressing both DNA repair and replication defects in mutations in genes uvsX, uvsY, 46, 47, and 59....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90225-3
更新日期:1984-09-01 00:00:00
abstract::Large numbers of VSV (LCMV) pseudotypes with the genomes of vesicular stomatitis virus (VSV) and the coat proteins of lymphocytic choriomeningitis virus (LCMV) were produced by infecting L cells first with LCMV and subsequently with VSV, the latter in the presence of tunicamycin. Separation by gradient centrifugation ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90007-2
更新日期:1984-08-01 00:00:00
abstract::A human B-lymphoid cell line, designated TG8, which does not express detectable levels of the EBV (Epstein-Barr virus)-associated nuclear antigen (EBNA), yet carries an average of one to two plasmid copies of the P3HR-1 EBV genome has been identified. TG8 can be superinfected by B95-8 EBV, resulting in up to 60-70% of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90014-x
更新日期:1984-08-01 00:00:00
abstract::Mixed infection of nonpermissive bacteria by amber mutant and wild-type T4 phage reduces the burst size in a manner that depends on the nature of the gene product eliminated by the mutation. While the genotypic proportions produced by such mixedly infected cells were directly proportional to those in the starting mixt...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90011-4
更新日期:1984-08-01 00:00:00
abstract::Coat protein-protected fragments of alfalfa mosaic virus RNA (AlMV-RNA) and tobacco streak virus RNA (TSV-RNA), which were isolated as described [D. Zuidema, M. F. A. Bierhuizen, B. J. C. Cornelissen, J. F. Bol, and E. M. J. Jaspars (1983)Virology, 125, 361-369], were tested for their ability to rebind AlMV coat prote...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90165-x
更新日期:1984-07-30 00:00:00
abstract::Monoclonal antibodies specific for eight antigenic sites on the G1 glycoprotein of La Crosse virus were studied in a kinetics of neutralization assay. When examined at the same protein concentration, there were differences in the rates and degree of neutralization induced by these antibodies. The maximum amount of neu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90163-6
更新日期:1984-07-30 00:00:00
abstract::Previously a new small subclass of SV40 large T antigen with a high-binding affinity to living target cells was characterized (J. Lange-Mutschler and R. Henning, 1983, Virology 127, 333-344.) In the present study the external binding process, particularly the tight linkage of T antigen to lipid of the target cells, wa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90176-4
更新日期:1984-07-30 00:00:00
abstract::Retroviruses can be grouped by viral interference measurements into classes which use common cell surface receptors. We previously tested a large number of isolates of mink cell focus-inducing (MCF) murine leukemia viruses (MuLVs), and reported that all of them share a distinct receptor on NIH/3T3 cells (A. Rein, Viro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90255-1
更新日期:1984-07-15 00:00:00
abstract::Vesicular stomatitis virus (VSV) isolated from two independently established lines of persistently infected mouse L cells expressed an altered phenotype of RNA synthesis at 37 degrees, the temperature at which the persistently infected cultures were maintained (T.K. Frey and J.S. Youngner, 1982, J. Virol. 44, 167-174)...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90260-5
更新日期:1984-07-15 00:00:00
abstract::Unlike cell lines transformed with Polyomaviruses, transformants derived by focus formation or colony formation in agarose medium after transfer into rat fibroblast cells (FR3T3 line) of Bovine Papilloma Type 1 (BPV1) DNA were consistently observed to grow poorly in suspension and to remain highly serum dependent for ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90196-x
更新日期:1984-06-01 00:00:00
abstract::After infection with mumps virus, cellular ribonucleic acid synthesis of a murine lymphoma cell line, EL4, was appreciably depressed. The inactivation of viral infectivity by ultraviolet irradiation or the treatment of cells with mouse interferon did not abolish the inhibiting effect, suggesting that virus replication...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90187-9
更新日期:1984-06-01 00:00:00
abstract::The purified genomic dsRNA of bluetongue virus type 1 (BTV 1) was separated into 10 size classes by polyacrylamide gel electrophoresis. These genome segments were recovered individually from the gel, denatured with methylmercuric hydroxide and translated in vitro in a rabbit reticulocyte lysate system. The virus-speci...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90131-4
更新日期:1984-05-01 00:00:00
abstract::Infections of nonpermissive ( sup0 ) Escherichia coli by T1 phage with amber mutations in either gene 3.5 or gene 4 exhibit a variety of defective phenotypes, including premature arrest of T1 DNA synthesis, failure to make concatemeric DNA, formation of an abnormal DNA replication intermediate, failure to package phag...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90130-2
更新日期:1984-05-01 00:00:00
abstract::Certain temperature-sensitive (ts) mutants of murine leukemia virus (MuLV) were observed to be defective in virus assembly. These mutants also accumulated intracellular core protein precursor, Pr65gag, at 39 degrees, the nonpermissive temperature. At 39 degrees, virions released from cells infected with the various ts...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90306-4
更新日期:1984-04-30 00:00:00
abstract::Terminally redundant linear proviral DNA of approximately 9.5 kb was the major unintegrated species recovered in the Hirt supernatant fraction of caprine synovial membrane cells infected with strain 75-G63 caprine arthritis-encephalitis virus. A physical map based on the cleavage sites of 13 restriction endonucleases ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90318-0
更新日期:1984-04-30 00:00:00
abstract::A human cytomegalovirus (HCMV)-induced polypeptide of 68,000 Da (p68) with protein kinase activity was identified using a monoclonal antibody (F6b) produced against HCMV-infected cell proteins. p68 was detected by immunoprecipitation from 3 to 120 hr after infection and was induced by several strains of human and not ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90295-2
更新日期:1984-04-30 00:00:00
abstract::Multiple RNA species which hybridize to a cloned cDNA probe of the turnip crinkle virus satellite RNA (S-TCV) are found both in virions and in plants infected with virus particles containing S-TCV. At least six of these RNAs appear to be multimeric forms of the satellite RNA based on their migration in denaturing gels...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90273-3
更新日期:1984-04-15 00:00:00
abstract::Rift Valley fever virus-induced protein synthesis was examined by polyacrylamide gel electrophoresis and fluorography. Five virus-induced polypeptides were detected, the nucleocapsid protein N, the nucleus-associated nonstructural protein NS1, the glycoproteins G1 and G2, and a protein of molecular weight 80K. The N, ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90277-0
更新日期:1984-04-15 00:00:00
abstract::Thymidine kinase-deficient syrian hamster cells were cotransfected with recombinant plasmids containing the thymidine kinase (TK) gene of Herpes Simplex Virus Type 1, and either intact or partially deleted SV40 T antigen-coding genes. The transformants were selected by their ability to grow in gHAT medium. After selec...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90287-3
更新日期:1984-04-15 00:00:00
abstract::The DNA extracted from bacteriophage 643, which infects group N strains of Streptococci, could be separated into four components by electrophoresis on agarose gels. Electron microscopy established the predominant form of the DNA to be a relaxed, circular molecule of molecular weight 14.9 X 10(6). The four species obse...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90406-9
更新日期:1984-03-01 00:00:00
abstract::Cytotoxic T-cell clones raised against X-31 (H3N2) influenza virus in C57BL/6 mice can be directed against an influenza A virus subtype specific determinant (1). A representative T-cell clone (A3.1) has been used in combination with a set of genetically typed recombinant viruses, to show that the A/PR/8/34 nucleoprote...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90413-6
更新日期:1984-03-01 00:00:00
abstract::The polycation DEAE-dextran increased the binding of VSV to BHK cells about fourfold over a wide range of VSV concentrations. The same proportion of bound virions was internalized by the cell in the presence or absence of DEAE-dextran. Viral primary RNA production was increased to the equivalent of a 4- to 4.5-fold in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90429-x
更新日期:1984-02-01 00:00:00
abstract::Monoclonal antibodies to avian sarcoma/leukosis virus-coded transforming, structural, and polymerase proteins have been developed. In this first communication, a monoclonal antibody to pp60src will be characterized. The antibody can bind to pp60src in radioimmunoassays, precipitates this antigen in protein kinase test...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90440-9
更新日期:1984-02-01 00:00:00
abstract::MuSV349 is a TB-cell line which produces infectious MuSV with little or no MuLV detectable by the XC assay. The apparent restriction of MuLV replication in MuSV349 cells was investigated. A replication-competent helper virus, with protein composition nearly identical to that of Mo-MuLV was isolated from the viruses pr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90043-6
更新日期:1984-01-30 00:00:00
abstract::An analysis has been made of variants found in preparations of the common (U1) strain of tobacco mosaic virus (TMV) which had been obtained after the inoculum had been passaged through a necrotic local lesion. These lesions were induced with a minimal number of virions (3000 or 30,000) per inoculation point. The relat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90097-7
更新日期:1984-01-15 00:00:00
abstract::Lipid binding properties which are dependent on exposure to acid pH are an important mechanism for the cellular entry pathway for some enveloped viruses and for other macromolecules. Cell-to-cell fusion can be used to demonstrate this function. La Crosse virus, a member of the family Bunyaviridae, fused BHK-21 cells f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90107-7
更新日期:1984-01-15 00:00:00
abstract::A 140,000-dalton disulfide-linked glycoprotein (gp140) specified by varicella-zoster virus (VZV) in infected cultured cells was identified and precipitated by two murine monoclonal antibodies (VZ-151 and VZ-158). When analyzed under reducing conditions by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, gp14...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90098-9
更新日期:1984-01-15 00:00:00
abstract::Hantaan virus, the prototype virus of hemorrhagic fever with renal syndrome, was examined for nucleic acid characteristics which would support its previously proposed inclusion in the virus family Bunyaviridae. Nucleocapsid RNA from Hantaan virions and a control bunyavirus were examined for ribonuclease A (RNase A) se...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90514-7
更新日期:1983-12-01 00:00:00